Description

8-Azidoethidium is a specialized, photoactivatable derivative of the classic nucleic acid stain ethidium bromide, designed for advanced molecular biology and biochemistry research. This compound matters for its unique ability to act as a photoaffinity labeling agent, enabling the covalent cross-linking and identification of nucleic acid binding sites upon UV irradiation. Researchers in academic, pharmaceutical, and biotechnology laboratories need it for probing DNA-protein interactions, studying enzyme mechanisms, and developing novel diagnostic assays.

Application

• Photoaffinity Labeling of Nucleic Acid-Protein Complexes: Used to covalently tag and identify proteins bound to DNA or RNA.
• Mechanistic Studies of Enzymes: Employed in research on topoisomerases, helicases, polymerases, and other nucleic acid-interacting enzymes.
• Fluorescence Quenching Assays: Serves as a probe in fluorescence-based studies of nucleic acid structure and dynamics.
• Development of Diagnostic Tools: Potential use in creating assays for detecting specific DNA sequences or protein binding events.
• Cross-linking Agent in Bioconjugation: Facilitates the stable attachment of nucleic acids to other molecules or surfaces for analytical purposes.
• Research on Mitochondrial DNA: As an ethidium derivative, it can be applied in studies related to mitochondrial function and genetics.

Basic Information

Product Name	8-Azidoethidium
CAS No.	69498-50-6
Molecular Formula	C21H19N7
Molecular Weight	369.43 g/mol
Synonyms	8-Azido-3,8-diamino-5-ethyl-6-phenylphenanthridinium; 8-Azido-3-amino-5-ethyl-6-phenylphenanthridinium; Ethidium Monoazide Azide; Photoactivatable Ethidium; Azidoethidium Bromide; 8-N3-Ethidium; Phenanthridinium, 8-azido-3-amino-5-ethyl-6-phenyl-
EINECS	Contact for details

Quality Control

Our 8-Azidoethidium is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation. A detailed Certificate of Analysis (COA) documenting identity, purity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to brown solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.