Description

3'-o-Methylcytidine-5'-Triphosphate Lithium Salt is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced biochemical and molecular biology applications. This compound is critical for researchers requiring precise control over nucleic acid structure and function, offering enhanced stability and specific incorporation properties. It is primarily utilized in the development of next-generation sequencing technologies, enzymatic synthesis of modified RNA, and as a vital tool in structural biology and drug discovery research.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for engineered polymerases in sequencing-by-synthesis platforms to study epigenetic modifications.
• Modified RNA Synthesis: Essential for the enzymatic in vitro transcription (IVT) of RNA containing site-specific 2'-O-methyl modifications, crucial for mRNA therapeutic development and basic research.
• Structural Biology Studies: Incorporated into oligonucleotides for X-ray crystallography and NMR studies to investigate RNA-protein interactions and ribozyme mechanisms.
• Diagnostic Assay Development: Serves as a key component in the design of sensitive molecular probes and primers for detecting specific RNA sequences.
• Enzyme Mechanism & Kinetics: Employed as a specialized substrate to study the specificity and catalytic activity of RNA polymerases and other nucleotide-processing enzymes.
• Antiviral & Anticancer Research: Investigated for its potential to inhibit viral polymerases or disrupt cellular RNA metabolism in targeted therapeutic strategies.

Basic Information

Product Name	3'-o-Methylcytidine-5'-Triphosphate Lithium Salt
CAS No.	69113-64-0
Molecular Formula	C11H18Li3N3O14P3
Molecular Weight	545.07 g/mol (anhydrous free acid basis)
Synonyms	2'-O-Methyl-CTP Lithium Salt; 3'-O-Methylcytidine 5'-(tetrahydrogen triphosphate) trilithium salt; m2'-O-CTP; 2'-O-Methylcytidine-5'-triphosphate trilithium salt; 2'-OMe-CTP Li Salt; 1-[(2R,3R,4R,5R)-5-(Hydroxy{hydroxy[hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl)-3,4-dihydroxyoxolan-2-yl]-4-amino-2-methoxypyrimidin-1-ium trilithium salt
EINECS	Contact for details

Quality Control

Our 3'-o-Methylcytidine-5'-Triphosphate Lithium Salt is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive life science research. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, and nuclease-free testing to guarantee suitability for enzymatic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). For long-term storage, keep under inert atmosphere or vacuum desiccation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Lithium Content (ICP)	Conforms to theoretical
Bioburden	Passes test (for nuclease-free grades)
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.