Description

Chymotrypsin Substrate I, Colorimetric is a specialized synthetic peptide derivative designed for the selective detection and quantification of chymotrypsin activity. This compound matters as a critical tool for researchers and quality control labs requiring precise, colorimetric measurement of enzymatic function. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for applications in enzyme kinetics studies, protease inhibitor screening, and biochemical assay development.

Application

• Enzyme Activity Assays: Quantitative measurement of chymotrypsin and related serine protease activity in research and diagnostic settings.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery for identifying and characterizing novel chymotrypsin inhibitors.
• Biochemical Research: Fundamental studies on protease kinetics, specificity, and mechanism of action.
• Diagnostic Kit Development: A key component in the formulation of colorimetric test kits for clinical and research laboratories.
• Quality Control: Monitoring chymotrypsin purity and activity in enzyme production and purification processes.
• Cell Biology Studies: Investigating the role of chymotrypsin-like proteases in cellular processes and signaling pathways.

Basic Information

Product Name	Chymotrypsin Substrate I, Colorimetric
CAS No.	68982-90-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide; Suc-AAPF-pNA; Suc-Ala-Ala-Pro-Phe-pNA; Chymotrypsin Substrate Suc-AAPF-pNA; N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide; AAPF-pNA; Protease Substrate I (Colorimetric)
EINECS	Contact for details

Quality Control

Our Chymotrypsin Substrate I, Colorimetric is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity verification, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in chymotrypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.