Description

N(4)-Anisoyl-5''-o-(4,4''-Dimethoxytrityl)-2''-Deoxycytidine) is a sophisticated nucleoside derivative, specifically a protected deoxycytidine monomer, which is critical for the controlled synthesis of oligonucleotides. This compound matters because it serves as a high-purity building block, enabling the precise assembly of DNA sequences for research and therapeutic development. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic laboratories engaged in antisense therapy, PCR, and next-generation sequencing applications. Its role is fundamental in ensuring the fidelity and efficiency of oligonucleotide manufacturing processes.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a key phosphoramidite precursor for the automated synthesis of DNA strands.
• Antisense Oligonucleotide (ASO) Development: For the research and production of therapeutic oligonucleotides targeting specific mRNA sequences.
• Polymerase Chain Reaction (PCR) Probe Synthesis: Used in the manufacture of labeled probes for diagnostic assays and genetic testing.
• Next-Generation Sequencing (NGS): As a reagent for constructing custom oligonucleotide libraries and primers.
• Gene Synthesis and Cloning: A fundamental component in the *de novo* synthesis of genes and gene fragments.
• Bioconjugation and Labeling: The protected structure allows for selective modification and attachment of reporter molecules.
• Academic and Pharmaceutical Research: For fundamental studies in nucleic acid chemistry, biochemistry, and drug discovery pipelines.

Basic Information

Product Name	N(4)-Anisoyl-5''-o-(4,4''-Dimethoxytrityl)-2''-Deoxycytidine)
CAS No.	68892-40-0
Molecular Formula	C43H41N3O8
Molecular Weight	727.81 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-anisoyl-2'-deoxycytidine; DMTr-dC(Anis) Amidite Precursor; N4-Anisoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine; N-Anisoyl-5'-O-DMT-2'-deoxycytidine; Protected Deoxycytidine Monomer; dC(Anis) DMT Protected Nucleoside; 2'-Deoxycytidine, N4-(4-methoxybenzoyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]- Oligonucleotide Synthesis Building Block
EINECS	Contact for details

Quality Control

Our N(4)-Anisoyl-5''-o-(4,4''-Dimethoxytrityl)-2''-Deoxycytidine) is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and the absence of critical impurities. Certificates of Analysis (COA) detailing all specifications are available upon request. Our production adheres to cGMP guidelines where applicable, supporting its use in pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider storage under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.