Description

Trifluoroacetyl-Dialanine-4-Nitroanilide is a specialized synthetic peptide derivative designed for advanced biochemical research and development. This compound is valued for its role as a chromogenic substrate in enzyme activity assays, particularly for proteases like elastase and chymotrypsin. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on enzymology and inhibitor screening.

Application

• Protease Enzyme Assays: Serves as a key chromogenic substrate for measuring the activity of serine proteases, including elastase, chymotrypsin, and related enzymes.
• Pharmaceutical Research: Used in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for therapeutic development.
• Diagnostic Development: Employed in the formulation of diagnostic kits for quantifying protease levels in clinical and research samples.
• Biochemical Mechanism Studies: Aids in kinetic studies and the elucidation of enzyme-substrate interaction mechanisms.
• Quality Control in Biomanufacturing: Applied in purity and activity testing of enzyme preparations used in industrial biocatalysis.

Basic Information

Product Name	Trifluoroacetyl-Dialanine-4-Nitroanilide
CAS No.	68739-16-2
Molecular Formula	C15H16F3N5O6
Molecular Weight	419.32 g/mol
Synonyms	N-(3-(2-((2,2,2-Trifluoroacetyl)amino)propanamido)propanoyl)-4-nitroaniline; TFA-Ala-Ala-pNA; Trifluoroacetyl-Ala-Ala-p-Nitroanilide; Ala-Ala-pNA (TFA); N-Succinyl-Ala-Ala-Ala-p-nitroanilide analog; Protease Substrate IV; Elastase Substrate; Chymotrypsin Substrate
EINECS	Contact for details

Quality Control

Every batch of Trifluoroacetyl-Dialanine-4-Nitroanilide is manufactured under strict quality management systems. We ensure high purity and batch-to-batch consistency through comprehensive analytical testing, including HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, identity, and related substances is provided with each shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.