Description

γ-L-Glutamyl-3-Carboxy-4-Nitroanilide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the accurate and sensitive detection of γ-glutamyltransferase (GGT) activity, a key diagnostic enzyme. It is primarily required by in-vitro diagnostic (IVD) kit manufacturers, clinical research laboratories, and pharmaceutical R&D departments for biochemical analysis and diagnostic applications.

Application

• Primary Substrate for GGT Assays: The core application is as a chromogenic reagent in clinical chemistry analyzers for the quantitative determination of γ-glutamyltransferase in human serum or plasma, aiding in the diagnosis of liver and biliary tract disorders.
• In-Vitro Diagnostic (IVD) Reagent Manufacturing: A key raw material for formulating ready-to-use liquid reagents, calibrators, and controls for automated clinical analyzers from major OEMs.
• Research & Development: Used in biochemical research to study enzyme kinetics, inhibition, and the role of GGT in various physiological and pathological processes, including oxidative stress and cancer biology.
• Quality Control in Pharmaceutical Manufacturing: Employed in enzymatic methods for quality control testing where GGT activity is a relevant parameter.
• High-Throughput Screening (HTS): Suitable for developing assays in drug discovery platforms to identify modulators of GGT activity.

Basic Information

Item	Details
Product Name	γ-L-Glutamyl-3-Carboxy-4-Nitroanilide
CAS No.	68699-78-5
Molecular Formula	C15H18N4O8
Molecular Weight	382.33 g/mol
Synonyms	L-γ-Glutamyl-3-carboxy-4-nitroanilide; γ-Glutamyl-3-carboxy-4-nitroanilide; GGT Substrate; 3-Carboxy-γ-L-glutamyl-4-nitroanilide; N-(3-Carboxy-4-nitrophenyl)-L-glutamine; Glutamine, N-[3-carboxy-4-nitrophenyl]-; GGPNA; L-γ-Glutamyl-3-Carboxy-4-Nitroanilide
EINECS	Contact for details

Quality Control

Our γ-L-Glutamyl-3-Carboxy-4-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR). We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specification results. Production can adhere to relevant ISO 13485 standards for IVD raw materials upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation and maintain assay performance.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Absorbance (Specific)	Conforms to standard
Solubility	Clear, yellow solution in specified buffer
Functional Performance (GGT Assay)	Meets reference standard activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.