Description

Disuccinimidyl Suberate is a homobifunctional, amine-reactive crosslinking agent featuring an extended 8-atom spacer arm. This compound is essential for creating stable covalent bonds between primary amines, facilitating the study of protein-protein interactions and the development of bioconjugates. It is a critical reagent for researchers and manufacturers in the fields of biochemistry, proteomics, and biopharmaceutical development who require reliable and efficient crosslinking solutions.

Application

• Protein Crosslinking & Conjugation: Primary reagent for covalently linking proteins, peptides, or antibodies to other biomolecules or solid supports.
• Bioconjugate Preparation: Used in the synthesis of antibody-drug conjugates (ADCs), enzyme complexes, and other therapeutic or diagnostic bioconjugates.
• Structural Biology Studies: Aids in the analysis of protein quaternary structure, subunit interactions, and complex assembly through crosslinking mass spectrometry (XL-MS).
• Immobilization Chemistry: Functionalizes surfaces (e.g., beads, chips, plates) with amine-containing ligands for assays, diagnostics, and affinity purification.
• Material Science: Crosslinking agent for modifying polymers or hydrogels with bioactive molecules to create functional biomaterials.
• Diagnostic Kit Development: Employed in the manufacturing of immunoassay components where stable covalent linkages are required.

Basic Information

Product Name	Disuccinimidyl Suberate
CAS No.	68528-80-3
Molecular Formula	C20H28N2O8
Molecular Weight	424.45 g/mol
Synonyms	DSS; Bis(succinimidyl) suberate; Suberic acid bis(N-hydroxysuccinimide ester); N,N'-Disuccinimidyl suberate; Disuccinimidyl octanedioate; BS3 (homobifunctional analog); 1,8-Bis(2,5-dioxopyrrolidin-1-yl)octane-1,8-dione; Bis(2,5-dioxopyrrolidin-1-yl) octanedioate
EINECS	Contact for details

Quality Control

Our Disuccinimidyl Suberate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research and bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability, consistency, and performance.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.