Description

(-)-3-Carboxy-2,2,5,5-Tetramethylpyrrolidinyl-1-Oxy is a stable nitroxide radical, specifically the carboxylic acid derivative of the widely used spin label TEMPO. This compound matters as a versatile and essential building block for synthesizing site-directed spin labels (SDSLs) used in Electron Paramagnetic Resonance (EPR) spectroscopy. Researchers and product developers in the life sciences and materials chemistry sectors need it to study protein dynamics, membrane fluidity, and polymer properties with high precision.

Application

• Site-Directed Spin Labeling (SDSL) for EPR/DEER spectroscopy to investigate protein structure, folding, and conformational changes.
• Bioconjugation via its carboxylic acid group to attach spin probes to peptides, proteins, antibodies, or other biomolecules.
• Polymer & Materials Science as a functional monomer or additive to study polymer dynamics, cross-linking, and radical processes.
• Membrane Biophysics for probing lipid bilayer properties and the interaction of membrane proteins.
• Antioxidant Research as a model compound for studying nitroxide-based radical scavenging mechanisms.
• MRI Contrast Agent Development for creating novel redox-sensitive or targeted imaging probes.

Basic Information

Product Name	(-)-3-Carboxy-2,2,5,5-Tetramethylpyrrolidinyl-1-Oxy
CAS No.	68354-47-2
Molecular Formula	C9H16NO3
Molecular Weight	186.23 g/mol
Synonyms	3-Carboxy-PROXYL; 3-Carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 3-Carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl; 3-Carboxy-TEMPO; 3-CP; (-)-3-Carboxy-PROXYL free radical; 1-Oxyl-2,2,5,5-tetramethyl-3-carboxypyrrolidine; 3-Carboxy-2,2,5,5-tetramethylpyrrolidin-1-oxyl
EINECS	Contact for details

Quality Control

Our (-)-3-Carboxy-2,2,5,5-Tetramethylpyrrolidinyl-1-Oxy is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is characterized by multiple analytical techniques, including HPLC, EPR, and NMR spectroscopy. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and radical content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). For long-term storage, consider keeping the material under an inert atmosphere (e.g., argon or nitrogen) to maintain optimal stability of the nitroxide radical.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (EPR)	Characteristic triplet signal
Purity (HPLC)	≥ 98.0%
Assay (Radical Content)	≥ 95%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.