Description

n-T-Boc-Leu-Gly-Arg p-Nitroanilide is a specialized synthetic peptide substrate designed for enzymatic research and biochemical assays. This compound is critical for studying the activity and inhibition of proteolytic enzymes, particularly serine proteases like thrombin and trypsin. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, where precise enzyme kinetics and substrate specificity are paramount for drug discovery and development.

Application

• Protease Activity Assays: Primary substrate for the spectrophotometric determination of serine protease activity, including thrombin, trypsin, and related enzymes.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A key reagent in kinetic studies to elucidate enzyme mechanisms, substrate specificity, and catalytic efficiency.
• Diagnostic Kit Manufacturing: Incorporated into in-vitro diagnostic (IVD) kits for clinical testing of coagulation factors and other protease-related biomarkers.
• Quality Control (QC) Testing: Employed in pharmaceutical QC labs to monitor the purity and potency of enzyme-based biopharmaceuticals.

Basic Information

Product Name	n-T-Boc-Leu-Gly-Arg p-Nitroanilide
CAS No.	68223-96-1
Molecular Formula	C24H38N8O7
Molecular Weight	550.61 g/mol
Synonyms	Boc-Leu-Gly-Arg-pNA; t-Boc-Leu-Gly-Arg p-Nitroanilide; N-α-t-Butyloxycarbonyl-Leucyl-Glycyl-Arginine 4-Nitroanilide; Boc-LGR-pNA; Thrombin Substrate I; Chromogenic Substrate for Thrombin; 68223-96-1; Leu-Gly-Arg pNA (Boc protected)
EINECS	Contact for details

Quality Control

Our n-T-Boc-Leu-Gly-Arg p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and batch-to-batch consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.