Description

Boc-Val-Leu-Gly-Arg-Pna is a protected tetrapeptide substrate featuring a para-nitroaniline (Pna) chromogenic leaving group. This compound is specifically designed for the sensitive and quantitative detection of trypsin-like serine protease activity, as the cleavage of the Arg-Pna bond releases the yellow-colored p-nitroaniline. It is an essential biochemical tool for researchers and developers in pharmaceutical R&D, enzymology studies, and high-throughput screening for protease inhibitors.

Application

• Protease Activity Assays: Primary substrate for kinetic studies and activity measurements of trypsin, thrombin, plasmin, and related serine proteases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitor candidates for therapeutic development.
• Enzymology Research: A critical reagent for investigating enzyme kinetics, specificity, and catalytic mechanisms of proteolytic enzymes.
• Diagnostic Kit Development: Serves as a key component in the development of colorimetric diagnostic tests for conditions involving abnormal protease activity.
• Biochemical Pathway Analysis: Utilized in cell-based and cell-free systems to study the role of specific proteases in signaling cascades and physiological processes.
• Quality Control in Biologics: Employed in purity and potency testing of enzyme-based biopharmaceutical products.

Basic Information

Item	Detail
Product Name	Boc-Val-Leu-Gly-Arg-Pna
CAS No.	68223-95-0
Molecular Formula	C30H48N10O9
Molecular Weight	692.77 g/mol
Synonyms	Boc-Val-Leu-Gly-Arg-pNA; t-Butyloxycarbonyl-Valyl-Leucyl-Glycyl-Arginine 4-Nitroanilide; BOC-VLG-R-PNA; N-α-t-Butyloxycarbonyl-L-valyl-L-leucyl-glycyl-L-arginine 4-nitroanilide; Protease Substrate V; Chromogenic Substrate for Trypsin; Serine Protease Substrate; 68223-95-0
EINECS	Contact for details

Quality Control

Every batch of Boc-Val-Leu-Gly-Arg-Pna is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive biochemical applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as purity (HPLC), identity (NMR, MS), and specific optical rotation. Our quality commitment supports research reproducibility and regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.