Description

p-Nitrophenyl Phosphate Di(Tris) Salt is a high-purity chromogenic substrate widely used in enzymatic assays. This compound is critical for the detection and quantification of alkaline phosphatase activity, a key enzyme in diagnostic and research applications. It is an essential reagent for clinical diagnostics, life science research, and biochemical manufacturing, where reliable and sensitive detection is paramount.

Application

• Clinical Diagnostic Kits: Primary substrate in ELISA and other immunoassays for detecting alkaline phosphatase-labeled antibodies.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and molecular biology protocols involving phosphatase activity.
• Pharmaceutical Quality Control: Employed in assays to monitor enzymatic activity during drug development and manufacturing processes.
• Food Safety Testing: Utilized in diagnostic kits for the detection of bacterial contamination (e.g., E. coli) which express alkaline phosphatase.
• In Vitro Diagnostics (IVD): A key component in automated clinical chemistry analyzers for various biomarker tests.
• Academic & Industrial Laboratories: Standard reagent for teaching and routine laboratory analysis of phosphatase enzymes.

Basic Information

Product Name	p-Nitrophenyl Phosphate Di(Tris) Salt
CAS No.	68189-42-4
Molecular Formula	C9H14NO8P • 2C4H11NO3
Molecular Weight	523.37 g/mol
Synonyms	PNPP Di(Tris) Salt; 4-Nitrophenyl Phosphate Di(Tris) Salt; pNPP Di(Tris) Salt; Phosphoric Acid 4-Nitrophenyl Ester Di(Tris) Salt; Tris p-Nitrophenyl Phosphate; ALP Substrate; Alkaline Phosphatase Substrate; Di(Tris) 4-Nitrophenyl Phosphate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl Phosphate Di(Tris) Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is tested to meet stringent specifications for purity, identity, and suitability for use as a diagnostic substrate. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (405 nm, Solution)	≤ 0.05
Solubility (1M Tris Buffer)	Clear, colorless solution
Enzymatic Suitability (Alkaline Phosphatase)	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.