Description

L-Glutamic Acid γ-(p-Nitroanilide) Hydrochloride is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of proteolytic enzyme activity, particularly for transglutaminases and other enzymes that cleave peptide bonds. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors seeking reliable and sensitive assay components.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric and spectrophotometric determination of transglutaminase (TGase) activity.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibitor screening for proteases and transglutaminases.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays during drug discovery processes targeting enzyme-related pathways.
• Diagnostic Reagent Manufacturing: A key component in the formulation of test kits for clinical and research diagnostics.
• Food Science & Quality Control: Applied in assays to measure transglutaminase activity in food processing, such as in meat and dairy product analysis.
• Academic Research: Utilized in university and institutional labs for studying enzyme mechanisms and protein cross-linking.

Basic Information

Product Name	L-Glutamic Acid γ-(p-Nitroanilide) Hydrochloride
CAS No.	67953-08-6
Molecular Formula	C11H14N4O5 • HCl
Molecular Weight	318.71 g/mol
Synonyms	L-Glutamic Acid γ-(p-Nitroanilide) Hydrochloride; N-α-CBZ-Gln-Gly; N-Carbobenzoxy-L-glutaminylglycine; CBZ-Gln-Gly; Z-Gln-Gly; Nα-Carbobenzyloxy-L-glutaminylglycine; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-5-((2,2,4,6,7-pentamethyl-2,3-dihydrobenzofuran-5-yl)sulfonyl)aminopentanoic acid (common name variant); Glutamyl-p-nitroanilide hydrochloride
EINECS	Contact for details

Quality Control

Our L-Glutamic Acid γ-(p-Nitroanilide) Hydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) documenting purity, assay, and related substances are available upon request to support your regulatory and quality assurance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.