Description

Affi-Gel Blue is a cross-linked agarose gel covalently coupled with the dye Cibacron Blue F3G-A, serving as a high-capacity affinity chromatography medium. This product is specifically engineered for the purification of proteins and enzymes, particularly those requiring nucleotide cofactors, such as dehydrogenases, kinases, and serum albumin. It is an essential tool for researchers and process development scientists in the biotechnology, pharmaceutical, and diagnostic industries seeking efficient, scalable purification solutions.

Application

• Purification of nucleotide-binding proteins and enzymes (e.g., dehydrogenases, kinases, albumin).
• Fractionation of blood plasma proteins, including serum albumin and coagulation factors.
• Intermediate purification and polishing steps in downstream bioprocessing for therapeutic proteins.
• Removal of specific contaminants or host cell proteins (HCP) from complex biological mixtures.
• Laboratory-scale protein isolation for research, assay development, and analytical purposes.
• Immobilization of enzymes for biocatalysis and industrial enzyme applications.

Basic Information

Product Name	Affi-Gel Blue
CAS No.	67701-58-0
Molecular Formula	N/A (Composite Material)
Molecular Weight	N/A (Composite Material)
Synonyms	Cibacron Blue 3G-A Agarose; Blue Sepharose; Cibacron Blue F3G-A Agarose; Dye-Ligand Chromatography Gel; Reactive Blue 2 Agarose; Affinity Gel Blue; Blue Agarose; Cibacron Blue-Agarose
EINECS	Contact for details

Quality Control

Our Affi-Gel Blue is manufactured under strict quality management systems. Each batch is tested for critical parameters including ligand density, binding capacity for a standard protein (e.g., Bovine Serum Albumin), particle size distribution, and flow characteristics. Certificates of Analysis (COA) detailing these specifications are provided to ensure reproducible performance and lot-to-lot consistency for your purification processes.

Storage

Preserve in a tightly closed container, protected from light. Store as a suspension in 20% ethanol or an appropriate preservative solution at 2-8°C. Do not freeze. Under these conditions, the product is stable for years. Always refer to the specific product data sheet for detailed storage instructions.

Specification

Item	Specification
Matrix	Cross-linked 4% or 6% Agarose Beads
Ligand	Cibacron Blue F3G-A
Ligand Density	~2 - 8 µmol dye per mL settled gel
Binding Capacity (BSA)	10 - 25 mg per mL settled gel
Particle Size	45 - 165 µm (wet)
Maximum Flow Rate	150 - 300 cm/hr
pH Stability	3 - 13 (long term); 2 - 14 (short term)
Chemical Stability	Stable in most aqueous buffers, detergents, and 8M urea. Avoid strong oxidizing agents.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.