Description

Glycylprolyl-4-Methylcoumaryl-7-Amide is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of dipeptidyl peptidase IV (DPP-IV) and related proline-specific peptidase activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric detection and quantification of Dipeptidyl Peptidase IV (DPP-IV, CD26) activity.
• Drug Discovery & Screening: Essential tool in high-throughput screening (HTS) platforms to identify and characterize DPP-IV inhibitors for therapeutic development (e.g., for type 2 diabetes).
• Biochemical Research: Used in kinetic studies to determine enzyme specificity, catalytic efficiency, and mechanism of action of proline-cleaving enzymes.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for measuring DPP-IV activity in biological samples.
• Cell Biology & Immunology: Applied in research investigating the role of cell surface DPP-IV in immune regulation, cancer biology, and signal transduction.
• Quality Control: Serves as a reference standard in pharmaceutical QC to ensure the potency and consistency of enzyme-based products.

Basic Information

Product Name	Glycylprolyl-4-Methylcoumaryl-7-Amide
CAS No.	67341-40-6
Molecular Formula	C₂₂H₂₆N₄O₅
Molecular Weight	426.47 g/mol
Synonyms	Gly-Pro-AMC; GP-AMC; Glycyl-Prolyl-7-Amido-4-Methylcoumarin; H-Gly-Pro-AMC; DPP-IV Substrate; Dipeptidyl Peptidase IV Fluorogenic Substrate; 7-Amido-4-methylcoumarin derivative of Glycyl-Proline; 4-Methylcoumaryl-7-amide Glycylproline
EINECS	Contact for details

Quality Control

Every batch of Glycylprolyl-4-Methylcoumaryl-7-Amide is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive research and diagnostic applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as purity (HPLC), identity (NMR, MS), and fluorogenic performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Fluorogenic Activity	Conforms to functional standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.