Description

(4-Methylumbelliferyl)-α-L-Iduronide Cyclohexylammonium Salt is a high-purity fluorogenic substrate specifically designed for enzymatic assays. This compound is critical for the sensitive and quantitative measurement of α-L-iduronidase activity, a key diagnostic enzyme. It is an essential reagent for research and diagnostic laboratories focused on lysosomal storage disorders, particularly Mucopolysaccharidosis I (MPS I, Hurler/Scheie syndrome).

Application

• Enzymatic Diagnostics: Primary substrate for the fluorometric assay of α-L-iduronidase activity in clinical diagnostics.
• Newborn Screening: Used in high-throughput screening programs to detect MPS I in dried blood spots.
• Biochemical Research: Tool for studying enzyme kinetics, inhibition, and the biochemical basis of lysosomal function and dysfunction.
• Therapeutic Development: Essential for in vitro testing and potency assays during the development of enzyme replacement therapies (ERT) and gene therapies for MPS I.
• Quality Control in Manufacturing: Used to standardize and validate the activity of recombinant α-L-iduronidase batches in pharmaceutical production.
• Carrier Testing: Assists in identifying carriers of MPS I by measuring intermediate enzyme activity levels.

Basic Information

Product Name	(4-Methylumbelliferyl)-α-L-Iduronide Cyclohexylammonium Salt
CAS No.	66895-33-8
Molecular Formula	C22H27NO10 • C6H13N
Molecular Weight	Contact for details
Synonyms	4-Methylumbelliferyl α-L-idopyranosiduronic acid cyclohexylammonium salt; 4-MU-α-L-Iduronide; α-L-Iduronidase substrate; MPS I Substrate; Fluorogenic iduronide substrate; 4-Methylumbelliferyl-α-L-iduronide; 4-MU-IdUA; Cyclohexylammonium 4-methylumbelliferyl-α-L-idopyranosiduronate
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under controlled conditions to ensure batch-to-batch consistency for critical diagnostic and research applications. Each lot undergoes comprehensive analysis, including HPLC purity assessment and functional activity validation in an enzymatic assay system. A detailed Certificate of Analysis (COA) is provided, documenting identity, purity, and performance specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Activity	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.