Description

Arachidonic Acid, [5,6,8,9,11,12,14,15-3H(N)]- is a high-specific-activity, tritium-labeled form of the essential polyunsaturated ω-6 fatty acid, arachidonic acid. This labeled compound is a critical tool for researchers requiring precise metabolic and biochemical pathway tracing, offering unparalleled sensitivity in detection. It is primarily utilized in advanced life science research, including studies on eicosanoid biosynthesis, lipid signaling, inflammation, and receptor pharmacology. The product is supplied with a defined specific activity and radiochemical purity to ensure reliable and reproducible experimental results.

Application

• Biochemical Research & Metabolic Pathway Tracing: Used as a radiolabeled tracer to study the metabolism, uptake, and distribution of arachidonic acid in cell cultures, tissues, and animal models.
• Eicosanoid Biosynthesis Studies: Essential for investigating the enzymatic conversion by cyclooxygenase (COX) and lipoxygenase (LOX) pathways into prostaglandins, thromboxanes, and leukotrienes.
• Lipid Signaling & Second Messenger Research: Employed to elucidate the role of arachidonic acid and its metabolites in cell signaling cascades, inflammation, and immune responses.
• Drug Discovery & Pharmacology: Serves as a key reagent in high-throughput screening (HTS) and mechanistic studies for developing anti-inflammatory and cardiovascular drugs that target arachidonic acid pathways.
• Receptor Binding & Enzyme Assays: Used in competitive binding assays and activity tests for enzymes like phospholipase A2 (PLA2) that release arachidonic acid from membrane phospholipids.
• Autoradiography & Imaging: Suitable for techniques requiring high-resolution detection, such as autoradiography, to localize arachidonic acid incorporation in biological samples.

Basic Information

Product Name	Arachidonic Acid, [5,6,8,9,11,12,14,15-3H(N)]-
CAS No.	66753-05-7
Molecular Formula	C20H28O2 (³H-labeled)
Molecular Weight	~304.4 g/mol (varies with specific activity)
Synonyms	(5Z,8Z,11Z,14Z)-5,8,11,14-Eicosatetraenoic acid (³H-labeled); All-cis-5,8,11,14-Eicosatetraenoic acid (³H-labeled); AA; ARA; [³H]Arachidonic Acid; Tritiated Arachidonic Acid; ³H-AA; 20:4(ω-6) (labeled)
EINECS	Contact for details

Quality Control

Every batch of our tritium-labeled Arachidonic Acid is manufactured and analyzed under controlled conditions to ensure the highest standards of radiochemical purity and specific activity. Quality is verified through advanced analytical techniques including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, radiochemical purity, chemical purity, solvent composition, and analytical chromatograms. Our quality system is designed to support GLP/GMP-compliant research.

Storage

Preserve in the original container as supplied. Store at or below -20°C. Protect from light. This product is easily oxidized (store under inert atmosphere). For long-term storage, solutions are recommended to be kept in airtight vials under an inert atmosphere such as argon or nitrogen. Always follow local regulations for the safe handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	Contact for details (typically 50-250 Ci/mmol)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Concentration	1.0 mCi/mL (Contact for other concentrations)
Solvent	Ethanol or Toluene/Ethanol (Specified on COA)
Identification (HPLC)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.