Description

L-Pyroglutamic Acid 4-Methyl-7-Coumarinylamide Hydrate is a specialized fluorogenic substrate and biochemical reagent. This compound is valued for its role as a sensitive and selective probe for enzymatic activity, particularly for proteases like pyroglutamyl-peptidase. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies, high-throughput screening, and the development of novel biochemical assays.

Application

• Enzyme Substrate for Protease Research: A key fluorogenic substrate for studying the activity and inhibition of pyroglutamyl aminopeptidase (PGP) and related enzymes.
• Biochemical Assay Development: Used in the design and optimization of sensitive fluorometric assays for enzyme kinetics and high-throughput screening (HTS) platforms.
• Diagnostic Reagent Manufacturing: Serves as a critical component in the production of diagnostic kits for measuring specific protease activities in clinical and research samples.
• Neuroscience and Cell Biology Studies: Applied in research investigating neuropeptide metabolism and intracellular proteolytic pathways.
• Pharmaceutical R&D: Utilized in drug discovery programs targeting proteases, aiding in the identification and characterization of potential therapeutic inhibitors.

Basic Information

Product Name	L-Pyroglutamic Acid 4-Methyl-7-Coumarinylamide Hydrate
CAS No.	66642-36-2
Molecular Formula	C₁₅H₁₄N₂O₅ • xH₂O
Molecular Weight	302.28 (Anhydrous basis)
Synonyms	L-Pyroglutamyl-4-methyl-7-coumarylamide; L-Pyroglutamic Acid 7-Amido-4-methylcoumarin; Pyr-AMC; PGP Substrate; L-Pyroglutamyl-AMC; 5-Oxo-L-proline 4-methyl-7-coumarinylamide; Glp-AMC; Fluorogenic pyroglutamyl peptidase substrate
EINECS	Contact for details

Quality Control

Our L-Pyroglutamic Acid 4-Methyl-7-Coumarinylamide Hydrate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and related substance profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.