Description

H-Gly-Gly-Pna Hcl is a high-purity, protected dipeptide derivative widely used as a critical building block in peptide synthesis and biochemical research. Its primary value lies in its role as a chromogenic substrate for the study of protease and peptidase enzyme activity, enabling precise kinetic assays and inhibitor screening. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors who require reliable, high-quality substrates for enzymatic studies and drug discovery programs.

Application

• Protease/Peptidase Substrate: Acts as a chromogenic p-nitroaniline (pNA) substrate for the quantitative determination of enzyme activity, particularly for aminopeptidases and other proteolytic enzymes.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and mechanism-of-action research in academic and industrial laboratories.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors as therapeutic candidates.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical analysis of specific enzyme levels.
• Peptide Synthesis Intermediate: Functions as a protected Gly-Gly dipeptide unit for the solid-phase or solution-phase synthesis of longer, more complex peptide sequences.

Basic Information

Product Name	H-Gly-Gly-Pna Hcl
CAS No.	66493-42-3
Molecular Formula	C11H15N5O4 • HCl
Molecular Weight	317.73 g/mol (Free base: 281.27 g/mol)
Synonyms	Glycyl-glycine p-nitroanilide hydrochloride; H-Gly-Gly-pNA • HCl; Gly-Gly-pNA hydrochloride; N-(2-Aminoacetyl)glycine 4-nitroanilide hydrochloride; GGPNA; Protease substrate GGpNA; L-Glycyl-glycine 4-nitroanilide hydrochloride; Peptide substrate for aminopeptidase
EINECS	Contact for details

Quality Control

Our H-Gly-Gly-Pna Hcl is manufactured under controlled conditions to ensure batch-to-batch consistency and high chemical purity suitable for sensitive biochemical applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided to guarantee compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in DMSO or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.