Description

H-Leu-Amc CAS NO 66447-31-2 is a fluorogenic peptide substrate widely used in biochemical and pharmaceutical research. This compound is critical for the sensitive and continuous assay of protease enzyme activity, particularly for enzymes like cathepsins and other cysteine proteases. It is an essential tool for researchers in drug discovery, enzymology, and diagnostic development, enabling the study of disease mechanisms and the screening of potential therapeutic inhibitors.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and quantification of leucine aminopeptidase and related protease activities.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Essential tool in enzymology studies to determine enzyme kinetics, specificity, and catalytic mechanisms.
• Diagnostic Development: Employed in the development of clinical diagnostic assays for conditions linked to abnormal protease activity.
• Cell Biology Studies: Used to monitor protease activity within live cells or cell lysates in studies of apoptosis, cancer, and other cellular processes.
• Quality Control in Biologics: Applied in the biopharmaceutical industry to assess the presence and activity of host cell proteases during production.

Basic Information

Product Name	H-Leu-Amc CAS NO 66447-31-2
CAS No.	66447-31-2
Molecular Formula	C19H27N3O3
Molecular Weight	345.44 g/mol
Synonyms	L-Leucine 7-amido-4-methylcoumarin; Leu-AMC; L-Leu-AMC; H-Leu-AMC; Leucine-7-amido-4-methylcoumarin; 7-Amido-4-methylcoumarin-Leucine; L-Leucine-7-amido-4-methylcoumarin; AMC-Leu
EINECS	Contact for details

Quality Control

Our H-Leu-Amc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment, guaranteeing batch-to-batch consistency and reliability for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.