Description

Z-Ala-Pro-Pna is a specialized peptide derivative, widely recognized as a chromogenic substrate for the detection and study of dipeptidyl peptidase IV (DPP-IV) activity. Its core value lies in enabling precise biochemical research and diagnostic assay development through its specific enzymatic cleavage, which releases a measurable colorimetric signal. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable tools for enzyme kinetics, inhibitor screening, and clinical testing.

Application

• Primary substrate for the colorimetric assay of Dipeptidyl Peptidase IV (DPP-IV/CD26) enzyme activity.
• Critical reagent in high-throughput screening (HTS) platforms for identifying and characterizing DPP-IV inhibitors, a major class of therapeutics for type 2 diabetes.
• Used in biochemical research to study the kinetics, specificity, and physiological role of DPP-IV in immunology, oncology, and endocrinology.
• Component in the development and quality control of in vitro diagnostic (IVD) kits for clinical laboratories.
• Tool compound for academic research investigating protease function and signal peptide processing.
• Reference standard for method validation and calibration in analytical laboratories.

Basic Information

Product Name	Z-Ala-Pro-Pna
CAS No.	66382-56-7
Molecular Formula	C20H22N4O6
Molecular Weight	414.41 g/mol
Synonyms	Z-Ala-Pro-pNA; Z-Ala-Pro-4-nitroanilide; N-Benzyloxycarbonyl-Alanyl-Prolyl-4-nitroanilide; Ala-Pro-pNA (Z-); DPP-IV Substrate; H-Ala-Pro-pNA (Z-); Chromogenic Substrate for DPP-IV; Z-Ala-Pro-4-NA
EINECS	Contact for details

Quality Control

Our Z-Ala-Pro-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We support compliance with relevant pharmacopeial guidelines for raw materials used in pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a dry environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol
Biological Activity	Suitable for DPP-IV assay (performance data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.