Description

n-Benzyloxycarbonylglycyl-Glycyl-Arginine-4-Methylcoumarinyl-7-Amide CAS NO 66216-78-2 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly trypsin and related enzymes. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for drug discovery, biochemical research, and enzyme characterization.

Application

• Protease Activity Assays: A highly sensitive fluorogenic substrate for the continuous kinetic measurement of trypsin, thrombin, and other serine proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: Essential tool for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial R&D settings.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for conditions related to abnormal protease activity.
• Quality Control in Biologics: Used to monitor and validate the activity and purity of enzyme preparations in manufacturing processes.

Basic Information

Product Name	n-Benzyloxycarbonylglycyl-Glycyl-Arginine-4-Methylcoumarinyl-7-Amide
CAS No.	66216-78-2
Molecular Formula	C32H41N9O7
Molecular Weight	663.73 g/mol
Synonyms	Z-Gly-Gly-Arg-AMC; Z-Gly-Gly-Arg-7-amido-4-methylcoumarin; Benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumaryl-7-amide; Cbz-Gly-Gly-Arg-AMC; Fluorogenic substrate for trypsin; Boc-Gly-Gly-Arg-AMC (common analog); Trypsin substrate Z-GGR-AMC; 7-Amido-4-methylcoumarin derivative of Z-Gly-Gly-Arg
EINECS	Contact for details

Quality Control

Our n-Benzyloxycarbonylglycyl-Glycyl-Arginine-4-Methylcoumarinyl-7-Amide is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to support your compliance and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Functional activity confirmed vs. reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.