Description

2,3-Diphenyl-5-Ethyltetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and electron acceptor in biochemical assays. Its core value lies in its ability to form intensely colored formazan dyes upon reduction, making it an essential reagent for measuring cell viability, metabolic activity, and dehydrogenase enzyme function. This compound is primarily needed by research institutions, pharmaceutical developers, and diagnostic kit manufacturers for applications in microbiology, cytotoxicity testing, and biochemical research.

Application

• Cell Viability and Proliferation Assays (e.g., MTT assay analogs): Used to quantify metabolically active cells in vitro.
• Microbiological Testing: Serves as an indicator for bacterial growth and metabolic activity in culture media.
• Dehydrogenase Enzyme Activity Studies: Acts as an electron acceptor in enzymatic reactions for research and diagnostic purposes.
• Cytotoxicity Screening: Employed in pharmaceutical and toxicological research to assess the toxic effects of compounds on living cells.
• Biochemical Research Reagent: Used in various laboratory protocols requiring a sensitive colorimetric detection method for redox reactions.
• Diagnostic Kit Formulation: A key component in the manufacture of clinical and research diagnostic kits for enzymatic analysis.

Basic Information

Product Name	2,3-Diphenyl-5-Ethyltetrazolium Chloride
CAS No.	66138-05-4
Molecular Formula	C21H19ClN4
Molecular Weight	362.85 g/mol
Synonyms	5-Ethyl-2,3-diphenyltetrazolium Chloride; DPTZ Chloride; Tetrazolium Red Chloride; 2,3-Diphenyl-5-ethyl-2H-tetrazolium Chloride; Ethyl Diphenyl Tetrazolium Chloride; NSC 15199; DPTTZ; DPTZ
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-Ethyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets high-purity research-grade standards. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in water, methanol, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.