Description

[125I]-Reverse Triiodothyronine CAS NO 66091-42-7 is a radiolabeled analog of the thyroid hormone reverse triiodothyronine (rT3), specifically iodinated with the radioactive isotope iodine-125. This compound is a critical tool for in vitro research and diagnostic assay development, enabling the study of thyroid hormone metabolism, receptor binding, and transport mechanisms. It is primarily utilized by pharmaceutical R&D teams, clinical diagnostics manufacturers, and academic researchers in endocrinology and nuclear medicine for radioimmunoassays (RIA), receptor studies, and tracer experiments.

Application

• Radioimmunoassay (RIA) Development and Calibration: Serves as the labeled tracer for the quantitative measurement of reverse triiodothyronine levels in biological samples.
• Thyroid Hormone Receptor Binding Studies: Used to investigate the affinity and kinetics of rT3 binding to nuclear thyroid hormone receptors and serum binding proteins (e.g., transthyretin, albumin).
• Metabolic Pathway Research: Acts as a tracer to study the peripheral metabolism, deiodination, and clearance pathways of thyroid hormones.
• Diagnostic Kit Manufacturing: A key component in the production of commercial in vitro diagnostic (IVD) test kits for thyroid function panels.
• Competitive Binding Assays: Employed in high-throughput screening (HTS) platforms to identify potential modulators of thyroid hormone action.
• Quality Control for Pharmaceutical Formulations: Used in analytical methods to assess batch consistency and stability of related pharmaceutical products.

Basic Information

Product Name	[125I]-Reverse Triiodothyronine
CAS No.	66091-42-7
Molecular Formula	C15H11I3NO4 *
Molecular Weight	Contact for details
Synonyms	3,3',5'-Triiodo-L-thyronine (radiolabeled); rT3 (125I); Reverse T3 Iodine-125; L-3,3',5'-Triiodothyronine (125I); (125I)-rT3; Radioiodinated Reverse Triiodothyronine; 125I-rT3; 3,3',5'-Triiodothyronine, L- (125I)
EINECS	Contact for details

* Formula shown for the unlabeled base compound. Specific activity and exact isotopic composition vary by batch.

Quality Control

Our [125I]-Reverse Triiodothyronine is produced and tested under stringent quality management systems. Each batch is characterized for critical parameters including radiochemical purity (typically >95% by HPLC), specific activity, and immunoreactivity to ensure performance in sensitive assays. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in the original lead-shielded container in a tightly closed secondary container, protected from all light exposure. Store at 2-8°C (refrigerated) or as specified on the product label and COA. Adhere to all local and international regulations for the safe storage and handling of radioactive materials. The product is strictly light-sensitive (protect from all light exposure).

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	Contact for details (typical range 2200 Ci/mmol)
Radionuclidic Purity	≥ 99% Iodine-125
Chemical Concentration	Contact for details
Solvent	Ethanol:Water or specified buffer
pH	As per COA
Expiry Date	Clearly marked on vial and COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.