Description

4-Methylumbelliferyl α-Mannobioside is a specialized fluorogenic substrate designed for the sensitive detection and measurement of α-mannosidase enzyme activity. This compound matters for researchers and diagnostic developers who require precise, high-throughput enzymatic assays. It is primarily needed by the life science research, pharmaceutical development, and clinical diagnostics industries for studying glycosidase function, lysosomal storage disorders, and high-content screening applications.

Application

• Enzymatic Assay Substrate: A critical fluorogenic reagent for the specific detection and kinetic analysis of α-mannosidase activity in vitro.
• Biomedical Research: Used in studies of glycoprotein processing, carbohydrate metabolism, and lysosomal biology.
• Diagnostic Development: Employed in the development of diagnostic kits for lysosomal storage disorders such as α-mannosidosis.
• High-Throughput Screening (HTS): Serves as a key substrate in HTS campaigns to identify modulators (inhibitors or activators) of α-mannosidase for drug discovery.
• Microbiology & Plant Biology: Applied in research to study mannosidase activity in microorganisms and plants related to cell wall metabolism and pathogenesis.
• Quality Control: Utilized as a reference standard in the quality control of enzyme preparations and diagnostic reagents.

Basic Information

Product Name	4-Methylumbelliferyl α-Mannobioside
CAS No.	66068-40-4
Molecular Formula	C22H28O15
Molecular Weight	532.45 g/mol
Synonyms	4-MU-α-Mannobioside; 4-Methylumbelliferyl-α-D-mannopyranosyl-(1→3)-D-mannopyranoside; α-Mannosidase Fluorogenic Substrate; 4-MU-α-Man-(1→3)-Man; 4-Methylumbelliferyl α-D-Mannobioside; 4-MU-α-Mannobioside; Fluorogenic α-Mannosidase Substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl α-Mannobioside is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A detailed Certificate of Analysis (COA) providing purity, assay, and impurity profile is supplied with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF
Fluorogenic Activity	Functional in standard α-mannosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.