Description

Bis-Peg1-Nhs Ester is a specialized heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester group and a thiol-reactive maleimide group, separated by a short polyethylene glycol (PEG) spacer. This compound is critical for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules while improving solubility and reducing aggregation. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation, where precise and reliable linkage chemistry is paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: Enables the site-specific coupling of different proteins or peptides for creating novel biologics and research tools.
• Diagnostic Reagent Preparation: Used to conjugate enzymes, fluorophores, or other reporter molecules to antibodies or antigens for ELISA, lateral flow assays, and other immunoassays.
• Surface Functionalization: For modifying nanoparticles, biosensors, or chromatography matrices with bioactive ligands.
• PEGylation Reagent: Facilitates the introduction of short PEG chains to biomolecules to enhance solubility and stability.
• Biomaterial Crosslinking: Employed in hydrogel formation and the development of advanced drug delivery systems.

Basic Information

Product Name	Bis-Peg1-Nhs Ester
CAS No.	65869-64-9
Molecular Formula	C₁₈H₂₀N₂O₉
Molecular Weight	408.36 g/mol
Synonyms	Bis-PEG1-NHS ester; 1,11-Bis-maleimidotriethyleneglycol; BM(PEG)1; BM(PEG)1 crosslinker; 1,1'-[1,11-dioxo-3,6,9-trioxa-1,10-diazau ndecamethylene]bis-1H-pyrrole-2,5-dione; 3,6,9-Trioxaundecanedioic acid, 1,11-bis(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-, bis(2,5-dioxo-1-pyrrolidinyl) ester; Maleimide-PEG1-NHS ester; NHS-PEG1-Maleimide
EINECS	Contact for details

Quality Control

Our Bis-Peg1-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR), to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.