Description

Nhs-Peo2-Nhs(Bs[Peg]2) CAS NO 65869-63-8 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring NHS ester and biotin groups, enabling efficient conjugation between amine-containing molecules and streptavidin/avidin systems. This compound is critical for creating stable, site-specific bioconjugates with preserved biological activity, which is essential for advanced diagnostic and therapeutic development. It is primarily needed by researchers and manufacturers in biopharmaceuticals, in vitro diagnostics (IVD), and proteomics for applications like antibody-drug conjugate (ADC) development, biosensor fabrication, and affinity purification.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a cleavable or non-cleavable spacer to connect cytotoxic payloads to monoclonal antibodies, enhancing therapeutic index and solubility.
• Diagnostic Assay Development: Used to immobilize proteins, peptides, or antibodies onto streptavidin-coated plates or beads for ELISA, lateral flow assays, and other biosensing platforms.
• Affinity Chromatography & Purification: Facilitates the biotinylation of ligands for capture and purification of target biomolecules using streptavidin resin.
• Cell Surface Labeling & Imaging: Enables the tagging of cell surface proteins with biotin for visualization or isolation via fluorescence-activated cell sorting (FACS) or microscopy.
• Protein & Peptide Modification: Allows for the site-specific introduction of biotin tags onto amine groups for studying protein-protein interactions, pull-down assays, and mass spectrometry.
• Nanoparticle Functionalization: Used to conjugate targeting ligands (e.g., antibodies, peptides) to nanoparticle surfaces for targeted drug delivery or imaging.
• Hydrogel & Biomaterial Crosslinking: Acts as a crosslinking agent to incorporate bioactive molecules into hydrogels for tissue engineering and controlled release applications.
• Oligonucleotide Conjugation: Facilitates the attachment of biotin to amine-modified oligonucleotides for detection and capture in molecular biology techniques.

Basic Information

Product Name	Nhs-Peo2-Nhs(Bs[Peg]2)
CAS No.	65869-63-8
Molecular Formula	C34H52N4O14S
Molecular Weight	772.87 g/mol
Synonyms	NHS-PEG2-Biotin; Biotin-PEG2-NHS ester; Biotinamidohexanoyl-PEG2-succinimidyl ester; Biotin-PEG2-NHS; Succinimidyl-[(N-biotinoyl-6-aminohexanoyl)aminoethoxyethoxy]acetate; Biotin (PEG)2 NHS Ester; Biotinylated PEG NHS ester; EZ-Link Biotin-PEG2-NHS Ester; 1-Biotinamido-3,6,9,12,15-pentaoxaheptadecan-17-oic Acid Succinimidyl Ester
EINECS	Contact for details

Quality Control

Our Nhs-Peo2-Nhs(Bs[Peg]2) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for bioconjugation research. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional validation is provided with every shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Functional Validation	Conjugation efficiency ≥ 90% (by test assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.