Description

2-Chloro-4-Nitrophenyl-α-D-Mannopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of α-mannosidase activity, a key enzyme in glycobiology research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying carbohydrate metabolism and enzyme function.

Application

• Enzymatic Substrate for α-Mannosidase: Primary use as a chromogenic substrate in biochemical assays to measure α-mannosidase enzyme activity.
• Glycobiology Research: Essential tool for studying glycosidase enzymes involved in glycoprotein processing and carbohydrate metabolism pathways.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for the detection of genetic disorders related to lysosomal storage diseases, such as α-mannosidosis.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of α-mannosidase for drug discovery.
• Quality Control in Bioprocessing: Applied in monitoring enzyme activity during the production and purification of recombinant glycoproteins.
• Microbiology Studies: Used to characterize bacterial or fungal glycosidases for microbiological research and identification.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-α-D-Mannopyranoside
CAS No.	65446-24-4
Molecular Formula	C12H14ClNO8
Molecular Weight	335.69 g/mol
Synonyms	4-Nitrophenyl 2-chloro-4-nitrophenyl α-D-mannopyranoside; 2-Chloro-4-nitrophenyl-α-D-mannoside; α-Mannosidase Substrate; CNP-α-D-Mannopyranoside; 2-Chloro-4-nitrophenyl α-D-mannoside; p-Nitrophenyl 2-chloro-4-nitrophenyl α-D-mannopyranoside; Chromogenic α-mannosidase substrate
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-α-D-Mannopyranoside is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and spectral analysis for identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.