Description

L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride is a high-purity, fluorogenic peptide substrate designed for sensitive biochemical research and diagnostic applications. This compound is critical for the specific detection and quantification of protease activity, particularly trypsin-like enzymes, due to its cleavable amide bond. It is an essential reagent for researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and proteomics, where precise enzyme kinetics and inhibitor screening are paramount.

Application

• Protease Activity Assays: Primary substrate for fluorometric assays targeting trypsin, thrombin, and other arginine-specific proteases.
• Drug Discovery & High-Throughput Screening (HTS): Used in screening libraries for novel protease inhibitors in pharmaceutical R&D.
• Clinical Diagnostics: Employed in the development of diagnostic kits for conditions related to abnormal protease activity (e.g., pancreatic function tests).
• Biochemical Research: A key tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Quality Control in Biologics: Used to monitor and validate the activity of enzyme-based therapeutics during manufacturing.

Basic Information

Product Name	L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	65286-27-3
Molecular Formula	C20H27N6O4 • HCl
Molecular Weight	458.93 g/mol
Synonyms	L-Arginine-7-amido-4-methylcoumarin HCl; Arg-AMC HCl; Boc-Gly-Arg-Arg-AMC (related precursor); 7-Amino-4-methylcoumarin derivative; Fluorogenic trypsin substrate; AMC-labeled arginine; Protease substrate 65286-27-3; L-Arg-AMC•HCl
EINECS	Contact for details

Quality Control

Our L-Arginine 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards required for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.