Description

n-Cbz-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like trypsin and thrombin. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for high-throughput screening, enzyme kinetics studies, and biochemical research.

Application

• Protease Activity Assays: A key fluorogenic substrate for the continuous, sensitive detection of trypsin, thrombin, and related serine proteases.
• High-Throughput Screening (HTS): Enables rapid screening of potential protease inhibitors or activators in drug discovery pipelines.
• Biochemical Research: Used in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) and elucidate catalytic mechanisms.
• Diagnostic Kit Development: Serves as a core component in the development of in-vitro diagnostic tests for conditions related to protease dysregulation.
• Quality Control in Biologics: Employed to monitor and quantify residual protease activity in downstream biopharmaceutical purification processes.
• Cell Biology Studies: Applied in assays to detect and localize protease activity within cellular and tissue samples.

Basic Information

Item	Detail
Product Name	n-Cbz-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	65147-22-0
Molecular Formula	C34H40N6O7 • HCl
Molecular Weight	689.19 g/mol (Free base: 652.73 g/mol)
Synonyms	Z-Phe-Arg-AMC • HCl; Cbz-Phe-Arg-7-amido-4-methylcoumarin Hydrochloride; Benzyloxycarbonyl-Phenylalanyl-Arginyl-7-Amido-4-Methylcoumarin Hydrochloride; AMC Substrate for Trypsin/Thrombin; Fluorogenic Peptide Substrate 65147-22-0; Boc-Phe-Arg-AMC (common alternate); Protease Substrate VII
EINECS	Contact for details

Quality Control

Our n-Cbz-Phe-Arg 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant quality guidelines suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.