Description

H-Pro-Phe-Arg-Amc Acetate Salt is a high-purity, fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly thrombin and related enzymes. It is essential for applications in pharmaceutical R&D, biochemical research, and diagnostic kit development where reliable and consistent substrate performance is non-negotiable.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of thrombin, trypsin, and other serine proteases.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) for drug discovery targeting coagulation pathways.
• Diagnostic Manufacturing: A key component in the production of clinical diagnostic kits for measuring blood coagulation parameters.
• Biochemical Research: Employed in enzyme kinetics studies, inhibitor characterization, and mechanistic studies of proteolytic enzymes.
• Quality Control (QC) Testing: Used in QC labs for batch testing of therapeutic enzymes and biopharmaceuticals.

Basic Information

Item	Details
Product Name	H-Pro-Phe-Arg-Amc Acetate Salt
CAS No.	65147-21-9
Molecular Formula	C31H41N9O6 • xC2H4O2
Molecular Weight	635.73 g/mol (Free base)
Synonyms	Boc-Pro-Phe-Arg-AMC Acetate; H-Pro-Phe-Arg-7-Amido-4-Methylcoumarin Acetate; Pro-Phe-Arg-AMC; Thrombin Substrate I (Fluorogenic); Boc-Pro-Phe-Arg-MCA Acetate; Z-Phe-Arg-AMC (related); Boc-Pro-Phe-Arg-AMC; Protease Fluorogenic Substrate
EINECS	Contact for details

Quality Control

Every batch of H-Pro-Phe-Arg-Amc Acetate Salt is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with rigorous internal specifications for identity, purity, and performance in fluorogenic assays. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting HPLC purity, water content, and functional activity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms (in thrombin assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.