Description

Gly-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic sectors for drug discovery, biochemical research, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for trypsin, thrombin, and other serine proteases, enabling real-time kinetic measurements.
• High-Throughput Screening (HTS): Used in pharmaceutical R&D for identifying and characterizing protease inhibitors as potential drug candidates.
• Biochemical Research: A key tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratories.
• Diagnostic Kit Development: Incorporated into clinical diagnostic assays for detecting specific protease biomarkers associated with diseases.
• Quality Control in Biologics: Employed to monitor and validate the activity and purity of enzyme-based therapeutics and reagents.

Basic Information

Product Name	Gly-Arg-7-Amino-4-Methylcoumarin
CAS No.	65147-19-5
Molecular Formula	C₂₂H₃₁N₇O₅
Molecular Weight	473.53 g/mol
Synonyms	Gly-Arg-AMC; Glycyl-L-arginyl-7-amido-4-methylcoumarin; Boc-Gly-Arg-AMC; L-Glycyl-L-arginine 7-amido-4-methylcoumarin; Fluorogenic substrate for trypsin; AMC substrate Gly-Arg; Boc-Gly-Arg-AMC acetate; Trypsin substrate IV
EINECS	Contact for details

Quality Control

Our Gly-Arg-7-Amino-4-Methylcoumarin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.