Description

Glt-Gly-Arg-Mca CAS NO 65147-16-2 is a specialized fluorogenic peptide substrate containing the 7-amino-4-methylcoumarin (AMC) leaving group. This compound is of significant value as a highly sensitive and selective tool for the study and quantification of protease activity, particularly thrombin and related enzymes. It is primarily needed by researchers and manufacturers in the fields of biochemical research, pharmaceutical development, and diagnostic assay production.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of thrombin and trypsin-like protease activity.
• Enzyme Kinetic Studies: Used to determine kinetic parameters (Km, Vmax, kcat) for target proteases in purified systems.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify and characterize novel protease inhibitors.
• Diagnostic Development: A key component in the development of sensitive fluorometric diagnostic tests for coagulation disorders.
• Biochemical Research: For studying protease function, regulation, and signaling pathways in various biological models.
• Quality Control in Biologics: Monitoring the presence and activity of specific proteases during biopharmaceutical manufacturing processes.

Basic Information

Product Name	Glt-Gly-Arg-Mca
CAS No.	65147-16-2
Molecular Formula	C₂₈H₄₁N₉O₉
Molecular Weight	623.69 g/mol
Synonyms	Boc-Glutaryl-Glycyl-Arginine-7-amido-4-methylcoumarin; Boc-Glu-Gly-Arg-AMC; Glutaryl-Gly-Arg-AMC; Boc-Glu-Gly-Arg-MCA; Thrombin Substrate I, Fluorogenic; Boc-E-G-R-MCA; 7-Amido-4-methylcoumarin derivative of Boc-Glu-Gly-Arg; Protease Fluorogenic Substrate
EINECS	Contact for details

Quality Control

Our Glt-Gly-Arg-Mca is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.