Description

Bolton-Hunter Reagent, [125I] is a specialized, radioiodinated compound widely used as a critical reagent for protein and peptide radiolabeling. Its primary value lies in enabling the introduction of a radioactive iodine-125 isotope into biomolecules under mild conditions, preserving their biological activity. This reagent is essential for researchers and professionals in pharmaceutical development, radioimmunoassay (RIA) manufacturing, and molecular biology research, facilitating studies in receptor binding, pharmacokinetics, and diagnostic imaging agent development.

Application

• Radioiodination of Proteins and Peptides: Primary application for labeling tyrosine residues or N-terminal amines without harsh oxidation conditions.
• Tracer Synthesis for Receptor Binding Assays: Creation of high-specific-activity radioligands for studying receptor density and affinity (e.g., GPCRs, hormone receptors).
• Immunoassay Development: Production of radioactive tracers for sensitive and quantitative radioimmunoassays (RIAs) and immunoradiometric assays (IRMAs).
• Metabolic and Pharmacokinetic Studies: Radiolabeling of therapeutic proteins or peptides to track distribution, metabolism, and clearance in preclinical models.
• Diagnostic Imaging Probe Development: A key intermediate in the synthesis of potential single-photon emission computed tomography (SPECT) imaging agents.
• Quality Control in Biopharmaceuticals: Used in internalization assays and other analytical methods to characterize biotherapeutic products.

Basic Information

Product Name	Bolton-Hunter Reagent, [125I]
CAS No.	65114-37-6
Molecular Formula	C14H16INO3 *
Molecular Weight	~389.09 g/mol (for non-radioactive form) *
Synonyms	N-Succinimidyl 3-(4-Hydroxy-3-[125I]iodophenyl)propionate
3-(4-Hydroxy-3-iodophenyl)propionic Acid N-Hydroxysuccinimide Ester, [125I]
Bolton and Hunter Reagent, [125I]
Iodine-125 Labeled Bolton-Hunter Reagent
SHPP, [125I] (Succinimidyl Hydroxyphenylpropionate)
Radioiodinated Bolton-Hunter Reagent
N-SHPP, [125I]
EINECS	Contact for details

* Formula and weight are for the core structure. Specific activity and exact concentration are batch-specific for the radioactive product.

Quality Control

Every batch of Bolton-Hunter Reagent, [125I] is manufactured and controlled under a strict quality management system. Release specifications include rigorous testing for radiochemical purity (typically >95% by HPLC), specific activity, and chemical purity. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing all analytical results to ensure consistency and suitability for your sensitive applications. Our quality commitment aligns with the needs of GMP-compliant research and development environments.

Storage

Preserve in the original container as supplied, tightly sealed, and protected from light. Store at or below -20°C upon receipt. Avoid repeated freeze-thaw cycles. This product is intended for use by trained personnel in appropriately licensed laboratories in accordance with all applicable radiation safety regulations.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Specific Activity	~2200 Ci/mmol (Batch-specific, stated on COA)
Radiochemical Purity (HPLC)	≥ 95%
Concentration	~1 mCi/mL in anhydrous benzene (or as specified)
Solvent	Anhydrous Benzene or other suitable anhydrous solvent
Expiry Date	Stated on label and COA (Typically 30-60 days from calibration date)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.