Description

Gly-Pro p-Nitroanilide p-Toluenesulfonate Salt is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the study of proteolytic enzymes, particularly dipeptidyl peptidases, due to its specific cleavage site. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers requiring high-purity, reliable substrates for kinetic studies and enzyme activity quantification.

Application

• Enzymatic Substrate for Dipeptidyl Peptidase IV (DPP-IV) Assays: A key reagent for measuring DPP-IV activity in research related to diabetes, immunology, and cancer.
• Biochemical Research & Protease Characterization: Used to study the kinetics, inhibition, and specificity of proline-specific peptidases.
• Pharmaceutical Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential DPP-IV inhibitor candidates.
• Diagnostic Reagent Manufacturing: Serves as a core component in the formulation of clinical diagnostic kits for enzyme activity tests.
• Academic Research: Utilized in university and institutional labs for fundamental studies on protein digestion and peptide metabolism.
• Quality Control in Biologics: Applied in monitoring enzyme activity during the production and purification of therapeutic proteins.

Basic Information

Product Name	Gly-Pro p-Nitroanilide p-Toluenesulfonate Salt
CAS No.	65096-46-0
Molecular Formula	C20H23N5O7S
Molecular Weight	477.49 g/mol
Synonyms	Glycyl-L-proline p-nitroanilide p-toluenesulfonate salt; H-Gly-Pro-pNA · Tos; Gly-Pro-pNA tosylate; DPP-IV Substrate; L-Glycyl-L-proline 4-nitroanilide 4-methylbenzenesulfonate; GPpNA tosylate; Dipeptidyl Peptidase IV Chromogenic Substrate; 4-Nitroaniline, N-[2-[[(2S)-1-oxo-2-pyrrolidinyl]methylamino]-2-oxoethyl]-, 4-methylbenzenesulfonate
EINECS	Contact for details

Quality Control

Our Gly-Pro p-Nitroanilide p-Toluenesulfonate Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation (IR, NMR), and residual solvent analysis to ensure it meets the stringent requirements of research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be kept under anhydrous conditions. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Heavy Metals	≤ 20 ppm
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.