Description

Z-Gly-Pro-Pna is a specialized peptide derivative and chromogenic substrate used extensively in biochemical research and pharmaceutical development. Its core value lies in its role as a selective and sensitive substrate for proteolytic enzymes, particularly dipeptidyl peptidases, enabling critical studies in enzyme kinetics and inhibitor screening. This compound is essential for researchers and manufacturers in the fields of protease research, drug discovery, and diagnostic assay development, where precise and reliable substrates are required.

Application

• Enzyme Assay Substrate: Primary substrate for the spectrophotometric detection and kinetic analysis of dipeptidyl peptidase IV (DPP-IV) and related proline-specific proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel inhibitors for metabolic and inflammatory diseases.
• Biochemical Research: A key tool for studying enzyme mechanism, specificity, and function in academic and industrial research laboratories.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for measuring specific protease activity in biological samples.
• Quality Control: Utilized in pharmaceutical quality control to test the activity of enzyme-based biopharmaceutical products.

Basic Information

Product Name	Z-Gly-Pro-Pna
CAS No.	65022-15-3
Molecular Formula	C21H22N4O6
Molecular Weight	426.43 g/mol
Synonyms	Z-Gly-Pro-pNA; Benzyloxycarbonyl-Glycyl-Prolyl-p-nitroanilide; Cbz-Gly-Pro-pNA; N-Carbobenzoxy-Glycyl-L-prolyl-4-nitroanilide; DPP-IV Substrate; Gly-Pro-pNA, Z-; H-Gly-Pro-pNA, Z-; Substrate for Dipeptidyl Peptidase IV
EINECS	Contact for details

Quality Control

Our Z-Gly-Pro-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing. A detailed Certificate of Analysis (COA) documenting identity, purity, and assay is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.