Description

4-(2-n-Maleimido)Methyl Benzoic Acid-Nhs is a heterobifunctional crosslinking reagent featuring both an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This compound matters for its ability to create stable, site-specific conjugates between proteins, peptides, and other biomolecules, enabling advanced research and diagnostic applications. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and the production of biosensors and immunoassay reagents.

Application

• Bioconjugation and Protein Labeling: For creating covalent linkages between amines and thiols on antibodies, enzymes, and other proteins.
• Antibody-Drug Conjugate (ADC) Development: As a critical linker molecule for attaching cytotoxic payloads to monoclonal antibodies.
• Surface Immobilization: For functionalizing gold surfaces, chips, or nanoparticles with biomolecules for biosensor and diagnostic assay development.
• Peptide Synthesis and Modification: Used to introduce functional handles or fluorescent tags onto synthetic peptides.
• Polymer and Hydrogel Functionalization: To incorporate bioactive ligands into biomaterial scaffolds for tissue engineering and drug delivery systems.
• Diagnostic Reagent Manufacturing: In the production of enzyme-linked immunosorbent assay (ELISA) kits and other immunoassay components.

Basic Information

Product Name	4-(2-n-Maleimido)Methyl Benzoic Acid-Nhs
CAS No.	64987-84-4
Molecular Formula	C16H14N2O6
Molecular Weight	330.29 g/mol
Synonyms	4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid N-hydroxysuccinimide ester; SMCC; Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylate; 4-Maleimidomethyl-1-cyclohexanecarboxylic acid N-hydroxysuccinimide ester; NHS-PEG4-Maleimide (related linker class); Heterobifunctional crosslinker SMCC; Succinimidyl trans-4-(maleimidylmethyl)cyclohexanecarboxylate; Amine-to-Thiol crosslinker
EINECS	Contact for details

Quality Control

Our 4-(2-n-Maleimido)Methyl Benzoic Acid-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.