Description

Val-Leu-Arg-p-Nitroanilide is a chromogenic peptide substrate designed for the selective detection and quantification of serine protease activity. This compound is of critical importance in biochemical research and diagnostic development, where precise enzyme kinetics are measured. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic reagent manufacturing industries.

Application

• Protease Activity Assays: Used as a specific substrate for measuring the enzymatic activity of trypsin, thrombin, and related serine proteases in research settings.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical analysis of blood coagulation factors and other protease-related pathways.
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Research: Facilitates kinetic studies and enzyme characterization in academic and industrial laboratories focused on proteolytic mechanisms.
• Quality Control Testing: Utilized in the QC of enzyme preparations and biopharmaceutical products to ensure batch-to-batch consistency and potency.

Basic Information

Product Name	Val-Leu-Arg-p-Nitroanilide
CAS No.	64816-14-4
Molecular Formula	C24H39N7O6
Molecular Weight	521.61 g/mol
Synonyms	Val-Leu-Arg-pNA; VLR-pNA; L-Valyl-L-leucyl-L-arginine 4-nitroanilide; Bz-Val-Leu-Arg-pNA (common historical name); S-2484 (substrate designation); Chromogenic Substrate for Trypsin; Thrombin Substrate; Protease Substrate VLRA-pNA
EINECS	Contact for details

Quality Control

Our Val-Leu-Arg-p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details
Extinction Coefficient	~ 10,000 M-1cm-1 at 405 nm (in assay buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.