Description

n-Benzoyl-Val-Gly-Arg p-Nitroanilide Hydrochloride is a chromogenic peptide substrate specifically designed for the selective and sensitive detection of serine proteases, most notably thrombin. This compound matters as a critical research and diagnostic reagent, enabling precise kinetic studies and activity assays for enzymes involved in coagulation, fibrinolysis, and other physiological pathways. It is primarily needed by biopharmaceutical R&D teams, clinical diagnostics manufacturers, and academic researchers focused on hemostasis, protease biochemistry, and drug discovery.

Application

• Thrombin Activity Assays: Primary substrate for the quantitative determination of thrombin activity in plasma samples, research samples, and purified enzyme preparations.
• Coagulation Pathway Research: Used in kinetic studies to investigate the activation and regulation of the blood coagulation cascade.
• Serine Protease Profiling: A tool for characterizing the substrate specificity and inhibition profiles of related trypsin-like serine proteases.
• Diagnostic Kit Development: A key component in the formulation of in-vitro diagnostic (IVD) kits for thrombotic and bleeding disorder assessments.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening potential anticoagulant or procoagulant drug candidates.
• Enzyme Kinetic Studies: Facilitates the determination of Michaelis-Menten constants (Km, Vmax) and inhibitor constants (Ki) for precise biochemical characterization.
• Quality Control in Biologics: Used to monitor the presence and activity of process-related proteases during the production of biopharmaceuticals.

Basic Information

Product Name	n-Benzoyl-Val-Gly-Arg p-Nitroanilide Hydrochloride
CAS No.	64815-80-1
Molecular Formula	C27H36ClN9O6
Molecular Weight	642.09 g/mol
Synonyms	Bz-Val-Gly-Arg-pNA · HCl; Benzoyl-Val-Gly-Arg-p-nitroanilide hydrochloride; Bz-VGR-pNA; Chromogenic Substrate for Thrombin; Thrombin Substrate I; Bz-VaI-GIy-Arg-pNA · HCl; (p-Nitroanilide) Benzoyl-L-valylglycyl-L-arginine hydrochloride; B-7632 (common catalog number)
EINECS	Contact for details

Quality Control

Our n-Benzoyl-Val-Gly-Arg p-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing against reference thrombin standards. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Functional Activity	≥ 95% of reference standard activity vs. thrombin
Solubility	Clear, colorless solution at 10 mg/mL in DMSO or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.