Description

Bz-Val-Gly-Arg-Pna CAS NO 64717-41-5 is a synthetic peptide substrate, specifically a chromogenic p-nitroanilide (pNA) derivative, widely used in biochemical research and diagnostic applications. Its core value lies in its specificity as a cleavage target for serine proteases, particularly thrombin and related enzymes, enabling precise measurement of enzymatic activity. This compound is essential for researchers and manufacturers in the fields of hemostasis research, pharmaceutical development, and in vitro diagnostic (IVD) kit production.

Application

• Biochemical Research: As a specific substrate for kinetic studies and inhibition assays of serine proteases like thrombin, trypsin, and factor Xa.
• Diagnostic Reagent Manufacturing: A key component in the formulation of chromogenic assay kits for clinical diagnostics, including coagulation tests and liver function panels.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel anticoagulant or anti-thrombotic drug candidates.
• Enzyme Characterization: Employed to determine enzyme specificity, catalytic efficiency (kcat/Km), and optimal reaction conditions in biochemical laboratories.
• Quality Control in Bioprocessing: Monitoring protease activity during upstream and downstream biomanufacturing processes to ensure product consistency.

Basic Information

Item	Details
Product Name	Bz-Val-Gly-Arg-Pna
CAS No.	64717-41-5
Molecular Formula	C27H35N9O6
Molecular Weight	581.63 g/mol
Synonyms	Benzoyl-Valyl-Glycyl-Arginine p-Nitroanilide; Bz-Val-Gly-Arg-pNA; Bz-VGR-pNA; Chromogenic Substrate S-2238 (H-D-Phe-Pip-Arg-pNA) Analog; Thrombin Substrate I (p-nitroanilide); Tosyl-Gly-Pro-Arg-pNA Related Compound; N-Benzoyl-L-valyl-glycyl-L-arginine 4-nitroanilide; Boc-Val-Gly-Arg-pNA Analog
EINECS	Contact for details

Quality Control

Our Bz-Val-Gly-Arg-Pna is produced under stringent quality management systems to ensure batch-to-batch consistency and reliability for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry for identity confirmation, and spectrophotometric assays for functional activity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.