Description

Porphobilinogen is a critical monopyrrole intermediate in the biosynthesis of tetrapyrroles, including heme, chlorophyll, and vitamin B12. Its precise chemical structure and high purity are essential for research and development in biochemistry and metabolic pathway analysis. This compound is primarily utilized by researchers in pharmaceutical development, agricultural science, and academic institutions studying porphyrias and related metabolic disorders.

Application

• Biochemical Research: Key substrate for studying the enzymatic steps in heme and chlorophyll biosynthesis pathways.
• Pharmaceutical Development: Used in the investigation of porphyria diseases and the development of diagnostic assays and potential therapeutic agents.
• Enzymology Studies: Serves as a standard or reagent in assays for enzymes like porphobilinogen deaminase (hydroxymethylbilane synthase).
• Metabolic Pathway Analysis: Essential for in vitro reconstruction and analysis of tetrapyrrole synthesis in both plant and animal systems.
• Academic & Industrial R&D: Fundamental building block in laboratories focused on bioinorganic chemistry and synthetic biology projects.
• Reference Standard: Used as a high-purity calibrant in analytical methods such as HPLC and mass spectrometry for metabolic profiling.

Basic Information

Product Name	Porphobilinogen
CAS No.	64176-86-9
Molecular Formula	C10H14N2O4
Molecular Weight	226.23 g/mol
Synonyms	3-[5-(Aminomethyl)-4-(carboxymethyl)-1H-pyrrol-3-yl]propanoic acid; PBG; Porphobilinogen; 1-Amino-5-oxo-4-(3-carboxypropyl)-2,3-dihydro-1H-pyrrole-2-acetic acid; 2,3-Dihydro-1H-pyrrol-1-ylacetic acid derivative; Uroporphyrinogen I synthase substrate; Hydroxymethylbilane synthase substrate
EINECS	Contact for details

Quality Control

Our Porphobilinogen is manufactured under controlled conditions to ensure high chemical purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is analyzed with modern analytical techniques, and a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided. We adhere to stringent internal quality standards to support the reliability required in pharmaceutical and biochemical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic and should be handled under dry conditions to prevent degradation. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Solubility	Soluble in water, dilute alkali; slightly soluble in most organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.