Description

Deoxycytidine Thiotriphosphate CAS NO 64145-29-5 is a modified nucleoside triphosphate analog where a sulfur atom replaces an oxygen atom in the phosphate backbone. This modification is critical for its primary role as a substrate in molecular biology techniques, particularly in next-generation sequencing (NGS) and polymerase-based applications. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require high-purity nucleotide analogs for advanced genomic research and development.

Application

• Next-Generation Sequencing (NGS): Used as a modified nucleotide in sequencing-by-synthesis platforms to enable unique detection signals or to terminate specific reactions.
• Polymerase Chain Reaction (PCR): Acts as a substrate for specialized DNA polymerases in applications requiring modified nucleotides.
• DNA/RNA Synthesis: Incorporated into oligonucleotides to confer nuclease resistance or for specific labeling strategies in probe development.
• Enzymatic Studies: Serves as a tool to study polymerase kinetics, fidelity, and mechanism due to its altered chemical properties.
• Diagnostic Assay Development: Utilized in the creation of molecular diagnostic tests and kits that rely on modified nucleotide incorporation.
• Biochemical Research: Employed in studies of nucleic acid metabolism, enzyme-substrate interactions, and structural biology.

Basic Information

Product Name	Deoxycytidine Thiotriphosphate
CAS No.	64145-29-5
Molecular Formula	C9H16N3O13P3S
Molecular Weight	523.22 g/mol
Synonyms	2'-Deoxycytidine-5'-triphosphate, thio-; dCTPαS; Deoxycytidine 5'-(trihydrogen triphosphate), 5'→5'-ester with 3'-deoxy-3'-thio-β-D-cytidine; Thio-dCTP; α-Thio-dCTP; Deoxycytidine 5'-[γ-thio]triphosphate; 2'-Deoxycytidine-5'-O-(1-thiotriphosphate)
EINECS	Contact for details

Quality Control

Our Deoxycytidine Thiotriphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive molecular biology applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and can provide material traceable to GMP principles for critical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.