Description

5-n-Acetylneuraminic Acid-5-Bromo-3-Indolyl-α-Ketoside is a specialized chromogenic substrate used for the detection of sialidase (neuraminidase) enzyme activity. This compound matters for its critical role in biochemical research and diagnostic applications, enabling the visualization and quantification of enzymatic processes. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies involved in microbiology, virology, and glycobiology studies.

Application

• Enzymatic Assay Substrate: Primary use as a chromogenic substrate for detecting and quantifying sialidase (neuraminidase) activity in vitro.
• Microbiological Research: Used in bacterial identification and characterization, particularly for pathogens like Clostridium perfringens and Vibrio cholerae that produce sialidases.
• Virology Studies: Applied in research on influenza viruses and other pathogens where neuraminidase is a key virulence factor and drug target.
• Diagnostic Kit Manufacturing: A key component in the formulation of culture media and test kits for clinical microbiology laboratories.
• Glycobiology Research: Tool for studying sialic acid metabolism, cell surface glycoconjugates, and carbohydrate-active enzymes.
• Quality Control in Pharma: Used in the development and validation of assays for products targeting neuraminidase.

Basic Information

Product Name	5-n-Acetylneuraminic Acid-5-Bromo-3-Indolyl-α-Ketoside
CAS No.	64122-65-2
Molecular Formula	C19H23BrN2O10
Molecular Weight	519.30 g/mol
Synonyms	5-Bromo-3-indolyl-α-ketoside of N-Acetylneuraminic Acid; 5-Bromo-3-indolyl-α-D-N-acetylneuraminic acid; X-α-Neu5Ac; 5-Br-Indolyl-α-Neu5Ac; Neuraminidase Substrate; Sialidase Chromogenic Substrate; N-Acetylneuraminic acid α-(5-bromo-3-indolyl) ketoside; 5-Bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminide (common related compound)
EINECS	Contact for details

Quality Control

Our 5-n-Acetylneuraminic Acid-5-Bromo-3-Indolyl-α-Ketoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed batch-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may affect performance. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Produces characteristic blue chromogen upon cleavage by sialidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.