Description

2'-Deoxyuridine, [5-3H] is a tritium-labeled nucleoside analog, a critical tool compound for biochemical and pharmacological research. Its radiolabeled form provides a powerful tracer for studying DNA synthesis, repair mechanisms, and the metabolic pathways of nucleoside-based therapeutics. This high-specific-activity material is essential for researchers in radiopharmaceutical development, oncology research, and molecular biology who require precise tracking of nucleotide incorporation and metabolism.

Application

• DNA Synthesis & Cell Proliferation Studies: Used as a tracer in thymidine incorporation assays to measure DNA synthesis rates and cell proliferation.
• Oncology & Anticancer Drug Research: Employed to investigate the mechanism of action and cellular uptake of fluoropyrimidine drugs like 5-fluorouracil (5-FU) and related prodrugs.
• Enzyme Kinetics & Inhibition Assays: Serves as a substrate for enzymes such as thymidine kinase and thymidylate synthase in kinetic and inhibition studies.
• Metabolic Pathway Tracing: Utilized to map the anabolic and catabolic pathways of pyrimidine nucleosides within cells and tissues.
• Autoradiography & Molecular Imaging: Acts as a radiolabel for in vitro and ex vivo autoradiography to visualize sites of active DNA synthesis.
• Radiopharmaceutical Precursor: Can be used in the research and development of novel tritium-labeled diagnostic or therapeutic agents.

Basic Information

Item	Detail
Product Name	2'-Deoxyuridine, [5-3H]
CAS No.	63844-76-8
Molecular Formula	C9H113HN2O5
Molecular Weight	Contact for details
Synonyms	5-Tritio-2'-deoxyuridine; [5-3H]Deoxyuridine; Thymidine, 2'-deoxy-5-tritio-; dUrd([5-3H]); 1-(2-Deoxy-β-D-ribofuranosyl)-5-tritiouracil; 2'-Deoxy-5-tritiouridine; Tritiated deoxyuridine; Radioactive deoxyuridine
EINECS	Contact for details

Quality Control

Every batch of 2'-Deoxyuridine, [5-3H] is manufactured and analyzed under strict quality systems. We guarantee high radiochemical purity and specific activity, with comprehensive testing including HPLC analysis for purity and radiometric detection for activity confirmation. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the specific activity, radiochemical purity, chemical purity, and solvent composition. Our quality commitment ensures data integrity for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C or below) in the original solvent as specified on the label and COA. For long-term storage, keep at -70°C to minimize radiolytic decomposition. The product is light-sensitive (store away from light). Always handle and store in accordance with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 98%
Concentration	1 mCi/mL (Solvent: Ethanol/Water mix)
Radioisotope	3H (Tritium)
Position of Label	5-Position of the uracil ring

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.