Description

5'-Dmt-5-Bromo-Du Phosphoramidite (Chiral) is a high-purity, chiral phosphoramidite monomer specifically engineered for solid-phase oligonucleotide synthesis. This compound is critical for the precise and efficient incorporation of modified nucleosides into DNA and RNA sequences, enabling advanced research and therapeutic development. It is an essential building block for pharmaceutical companies, biotechnology research labs, and diagnostic developers working on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies.

Application

• Oligonucleotide Synthesis: Key monomer for the automated solid-phase synthesis of DNA and RNA strands containing 5-bromodeoxyuridine (5-BrdU) modifications.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotides targeting specific genes for the treatment of genetic disorders, cancers, and viral infections.
• Diagnostic Probe Development: Manufacturing of labeled probes for fluorescence in situ hybridization (FISH), microarray analysis, and other molecular diagnostic assays.
• Aptamer Selection (SELEX): Construction of chemically modified nucleic acid libraries to identify high-affinity binders for therapeutic and sensor applications.
• Structural Biology Studies: Incorporation of heavy atom (bromine) labels via 5-BrdU for phase determination in X-ray crystallography of nucleic acid complexes.
• Photocrosslinking & Bioconjugation: Utilized to create photoactivatable or chemically reactive sites within oligonucleotides for studying nucleic acid-protein interactions or creating conjugates.

Basic Information

Product Name	5'-Dmt-5-Bromo-Du Phosphoramidite (Chiral)
CAS No.	63660-21-9
Molecular Formula	C37H42BrN4O7P
Molecular Weight	789.64 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-5-bromo-2'-deoxyuridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5-Bromo-2'-deoxyuridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 5-BrdU Phosphoramidite; 5-Bromo-dU Phosphoramidite; DMT-5-Bromo-dU-CE Phosphoramidite; 5-Bromo-2'-deoxyuridine 3'-phosphoramidite, DMT-protected.
EINECS	Contact for details

Quality Control

Every batch of 5'-Dmt-5-Bromo-Du Phosphoramidite (Chiral) is manufactured and tested under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and moisture content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.