Description

Valyl-Leucyl-Lysine 4-Nitroanilide is a synthetic tripeptide substrate featuring a chromogenic 4-nitroaniline leaving group. This compound is specifically designed for the sensitive and quantitative detection of proteolytic enzyme activity, particularly that of trypsin and related serine proteases. It is an essential research-grade biochemical for scientists and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic reagent manufacturing sectors.

Application

• Enzyme Activity Assays: Primary substrate for the kinetic and endpoint analysis of trypsin, thrombin, plasmin, and other serine proteases.
• Biochemical Research: Used in mechanistic studies of enzyme inhibition, substrate specificity profiling, and protease signaling pathway analysis.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring pancreatic function and coagulation factors.
• Pharmaceutical Quality Control (QC): Employed in purity and potency testing of enzyme-based biopharmaceuticals during production and batch release.
• Protease Inhibitor Screening: Serves as a standard substrate in high-throughput screening (HTS) platforms to identify and characterize novel therapeutic protease inhibitors.

Basic Information

Product Name	Valyl-Leucyl-Lysine 4-Nitroanilide
CAS No.	63589-93-5
Molecular Formula	C26H43N7O6
Molecular Weight	549.67 g/mol
Synonyms	Val-Leu-Lys-pNA; VLL-pNA; N-Valyl-N'-leucyl-N''-lysyl-4-nitroaniline; Trypsin Substrate VLL-pNA; Boc-Val-Leu-Lys-pNA; Protease Substrate 63589-93-5; Nα-Benzyloxycarbonyl-Val-Leu-Lys-p-nitroanilide (related protected form)
EINECS	Contact for details

Quality Control

Every batch of Valyl-Leucyl-Lysine 4-Nitroanilide is manufactured and tested under a strict quality management system. We guarantee high chemical purity and consistent enzymatic performance suitable for critical research and diagnostic applications. Comprehensive analytical testing, including HPLC for purity and functional activity assays, is performed. A detailed Certificate of Analysis (COA) documenting identity, purity, assay, and related substances is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.