Description

4-Azidobenzoyl-Coenzyme A is a specialized biochemical reagent featuring a photoreactive azido group. This compound is critical for photoaffinity labeling studies, enabling the covalent cross-linking and identification of protein binding sites and enzyme active centers. It is an essential tool for researchers in biochemistry, enzymology, and pharmaceutical development who require precise molecular probes for studying CoA-dependent enzymatic mechanisms.

Application

• Photoaffinity Labeling Probe: Used to covalently tag and identify binding sites of CoA-dependent enzymes and proteins.
• Enzyme Mechanism Studies: Essential for investigating the catalytic pathways and substrate recognition of acyl-CoA utilizing enzymes.
• Proteomics Research: Employed in activity-based protein profiling (ABPP) to discover and characterize novel enzyme targets.
• Bioconjugation and Cross-linking: Serves as a key reagent for creating stable conjugates between biomolecules under controlled light activation.
• Drug Discovery Target Validation: Facilitates the study of enzyme-inhibitor interactions for validating potential therapeutic targets.
• Metabolic Pathway Analysis: Aids in tracing and understanding specific steps in fatty acid and polyketide biosynthesis pathways.

Basic Information

Product Name	4-Azidobenzoyl-Coenzyme A
CAS No.	63550-94-7
Molecular Formula	C29H41N8O17P3S
Molecular Weight	898.66 g/mol
Synonyms	4-Azidobenzoyl-CoA; p-Azidobenzoyl Coenzyme A; N-[(4-Azidobenzoyl)-β-alanyl]-CoA; 4-Azidobenzoylcoenzyme A; Azidobenzoyl-CoA; Coenzyme A, S-[4-azidobenzoyl] derivative; Photoactivatable CoA derivative; AB-CoA
EINECS	Contact for details

Quality Control

Our 4-Azidobenzoyl-Coenzyme A is produced under stringent quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is analyzed by HPLC and NMR to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store as supplied and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.