Description

p-Nitrophenyl Phosphate Di(2-Amino-2-Ethyl-1,3-Propanediol) Salt is a high-purity, stabilized chromogenic substrate widely used in enzymatic assays. This compound is critical for providing reliable, colorimetric detection of alkaline phosphatase activity, a key marker in numerous diagnostic and research protocols. It is an essential reagent for manufacturers and researchers in the clinical diagnostics, life sciences, and biotechnology sectors requiring precise and reproducible results.

Application

• Clinical Diagnostic Kits: Primary substrate for detecting alkaline phosphatase (ALP) in ELISA, immunoblotting, and automated clinical chemistry analyzers.
• Molecular Biology Research: Used in nucleic acid hybridization detection systems (e.g., Southern, Northern, and Western blots) employing alkaline phosphatase-conjugated probes.
• Pharmaceutical Quality Control: Employed in enzymatic assays to monitor phosphatase activity during drug development and manufacturing processes.
• Food Safety Testing: Utilized in microbiological and enzymatic test kits for pathogen detection and food quality assessment.
• Biochemical Reagent Production: Serves as a key raw material for formulating ready-to-use substrate buffers and detection kits.

Basic Information

Product Name	p-Nitrophenyl Phosphate Di(2-Amino-2-Ethyl-1,3-Propanediol) Salt
CAS No.	62796-28-5
Molecular Formula	C17H32N4O10P
Molecular Weight	492.44 g/mol
Synonyms	PNPP Di(2-Amino-2-Ethyl-1,3-Propanediol) Salt; 4-Nitrophenyl Phosphate Di(AMP) Salt; p-NPP Di(AMP) Salt; Phosphoric Acid 4-Nitrophenyl Ester Di(2-Amino-2-Ethyl-1,3-Propanediol) Salt; Alkaline Phosphatase Substrate; PNPP; 4-Nitrophenyl Phosphate Disodium Salt Alternative; Diagnostic Grade PNPP
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl Phosphate Di(2-Amino-2-Ethyl-1,3-Propanediol) Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive diagnostic applications. Each lot is tested to meet stringent specifications for identity, purity, and functional performance. Certificates of Analysis (COA) detailing assay, impurity profile, and suitability for use are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility (1% in Water)	Clear, colorless to faint yellow solution
Functional Test (ALP Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.