Description

H-Leu-Amc Hcl CAS NO 62480-44-8 is a protected amino acid derivative, specifically the hydrochloride salt of L-Leucine 7-amido-4-methylcoumarin. This compound is a critical fluorogenic substrate and biochemical tool used extensively in enzymatic research and assay development. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for studying protease activity, particularly that of aminopeptidases and other hydrolytic enzymes.

Application

• Protease & Enzyme Assay Development: As a fluorogenic substrate for high-throughput screening (HTS) and kinetic studies of leucine aminopeptidase and related proteases.
• Biochemical Research: A key reagent for investigating enzyme mechanisms, inhibition, and specificity in academic and industrial R&D laboratories.
• Pharmaceutical Discovery: Used in drug discovery programs to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Diagnostic Kit Manufacturing: Incorporated into diagnostic assays and kits for the detection and quantification of specific enzymatic activities in clinical samples.
• Cell Biology Studies: Employed in live-cell imaging and assays to monitor protease activity within cellular pathways.
• Quality Control in Bioprocessing: Used to assay the activity and consistency of enzyme preparations used in industrial bioprocesses.

Basic Information

Product Name	H-Leu-Amc Hcl
CAS No.	62480-44-8
Molecular Formula	C₁₆H₂₁ClN₂O₃
Molecular Weight	324.80 g/mol
Synonyms	L-Leucine 7-amido-4-methylcoumarin hydrochloride; Leu-AMC HCl; H-Leu-AMC•HCl; L-Leucyl-7-amido-4-methylcoumarin hydrochloride; 7-(L-Leucylamido)-4-methylcoumarin hydrochloride; Aminopeptidase Substrate (Leu-AMC); Fluorogenic Leucine Substrate; Leucine-7-amido-4-methylcoumarin HCl
EINECS	Contact for details

Quality Control

Our H-Leu-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and optical rotation analysis to ensure chiral integrity and batch-to-batch consistency. Certificates of Analysis (COA) detailing all specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.