Description

H-D-Pro-Phe-Arg-Pna 2 Hcl is a high-purity, chromogenic peptide substrate specifically designed for biochemical research and diagnostic applications. This compound is critical for the precise and sensitive detection of serine protease activity, particularly thrombin and related enzymes. It is an essential reagent for pharmaceutical R&D, clinical diagnostics, and academic research in fields like hematology and enzymology.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric or fluorometric determination of thrombin, trypsin, and other serine proteases.
• Diagnostic Kit Manufacturing: A key component in the production of in-vitro diagnostic (IVD) kits for blood coagulation disorders and thrombotic risk assessment.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to identify and characterize novel protease inhibitors as potential drug candidates.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, substrate specificity, and inhibition mechanisms in academic and industrial laboratories.
• Quality Control in Biologics: Employed to monitor and quantify residual protease activity in the downstream purification of therapeutic proteins and antibodies.

Basic Information

Product Name	H-D-Pro-Phe-Arg-Pna 2 Hcl
CAS No.	62354-56-7
Molecular Formula	C30H42Cl2N10O5
Molecular Weight	693.63 g/mol
Synonyms	H-D-Pro-Phe-Arg-p-Nitroanilide Dihydrochloride; D-Phenylalanyl-L-prolyl-L-arginine 4-nitroanilide dihydrochloride; Thrombin Substrate I; Chromogenic Substrate S-2238; Tosyl-Gly-Pro-Arg-pNA·2HCl; Bz-Phe-Val-Arg-pNA·2HCl (common related substrate); H-D-Pro-Phe-Arg-pNA·2HCl; BOC-D-Phe-Pro-Arg-pNA (protected analog)
EINECS	Contact for details

Quality Control

Every batch of H-D-Pro-Phe-Arg-Pna 2 Hcl is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We guarantee high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. A detailed Certificate of Analysis (COA) providing HPLC purity, absorbance characteristics, water content (KF), and identity confirmation (MS, NMR) is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (Max)	310 - 320 nm (in suitable solvent)
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.