Description

D-Val-Leu-Lys p-Nitroanilide Dihydrochloride is a high-purity, chromogenic peptide substrate specifically designed for enzymatic research and diagnostic applications. This compound is critical for the precise measurement of protease activity, particularly for enzymes like plasmin and trypsin, where it serves as a sensitive and specific substrate. It is an essential reagent for researchers and manufacturers in the fields of biochemical research, pharmaceutical development, and in vitro diagnostic (IVD) kit production who require reliable and consistent performance in their assays.

Application

• Biochemical Research: Used as a specific chromogenic substrate for kinetic studies and inhibition assays of serine proteases like plasmin, trypsin, and related enzymes.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic applications.
• In Vitro Diagnostic (IVD) Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring fibrinolytic activity or specific protease levels in biological samples.
• Enzyme Characterization: Facilitates the determination of kinetic parameters (Km, Vmax) and specificity constants for purified proteases.
• Quality Control in Biologics: Used to monitor and quantify residual protease activity in downstream bioprocessing of therapeutic proteins.
• Academic Research: Supports fundamental studies in hemostasis, thrombosis, cell signaling, and other protease-mediated physiological and pathological processes.

Basic Information

Product Name	D-Val-Leu-Lys p-Nitroanilide Dihydrochloride
CAS No.	62354-43-2
Molecular Formula	C24H40Cl2N8O6
Molecular Weight	607.54 g/mol
Synonyms	D-Valyl-L-leucyl-L-lysine 4-nitroanilide dihydrochloride; H-D-Val-Leu-Lys-pNA•2HCl; S-2251 Substrate; Chromogenic Substrate S-2251; Plasmin Substrate; D-Val-Leu-Lys-pNA; Val-Leu-Lys p-nitroanilide dihydrochloride; Chromozym PL
EINECS	Contact for details

Quality Control

Every batch of D-Val-Leu-Lys p-Nitroanilide Dihydrochloride is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with stringent specifications for identity, purity, and performance suitable for research and diagnostic applications. A detailed Certificate of Analysis (COA) providing HPLC purity, absorbance characteristics, and functional activity data is supplied with each shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (λ max)	310 - 320 nm
Functional Activity	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.